ZFIN Image: Saraceni et al., 2016, Fig. 5

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Figure Caption

Fig. 5

(A) In vivo imaging of neutrophils from Tg(mpx:GFP+/+) larvae after injury and bath infection with A. hydrophila (10⁸ CFU/mL). Recruitment of neutrophils to the site of the injury 1 h after the bacterial infection. Control animals were injured but not infected. 3D reconstruction of a selected site in the infected sample showing the positions of the bacteria (red) inside the neutrophils (green). (B) Relative quantification of neutrophils in the site of injury. Results are representative of three independent experiments (n = 10-15 larvae each; ANOVA; *P < 0.05, **P < 0.01). (C) Expression of IL-1β and TNFα in injured larvae infected by bath immersion (10⁸ CFU/mL) at 1, 3, and 5 hpi. Results of three independent experimental infections (n = 30) expressed as the mean ± standard error (ANOVA and Tukey HSD test; *P < 0.05, **P < 0.01).

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Expression / Labeling details

Acknowledgments

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