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Fig. 4
Whole-mount in situ labeling of embryos visualizing the forebrain expression domains of zotx-2 (14 hour), zash-1b (12 hour), and shh (14 hour) of wild-type (A-C) and mbl (D-F) embryos. (A,D) Lateral view of the head showing an expansion of the zotx-2 expression domain anteriorly in mbl (arrowhead points to the epiphysis anlage in wild type). (B,E) Lateral view of the head showing the absence of the telencephalic expression domain of zash-1b in mbl. (C,F) Lateral view of the head showing an expansion of the hypothalamic (asterisk) and/or diencephalic expression domain (arrowhead) of shh in mbl. Anterior to the left.