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Fig. 2
RT-PCR analyses of the temporal expression patterns of wnt8, wnt8b and max during normal embryogenesis. RT-PCR was performed on cDNAs synthesized from RNA isolated from select developmental stages. Lanes designated plus or minus refer to the presence or absence (control) of reverse transcriptase in the firststrand cDNA synthesis. (A) wnt8 expression, first detected at the 2- to 4-cell stage and corresponding to the presence of maternal transcripts, continues throughout embryogenesis. (B) wnt8b transcripts are not maternal and, unlike wnt8, they are first detected at a time corresponding to the late gastrula stage. (C) The relative consistency in the max signals throughout embryogenesis indicates that approximate equivalent amounts of cDNAs were being assayed in the wnt8 and wnt8b PCR analyses.