ZFIN Image: Talbot et al., 2016, Fig. 6

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Figure Caption

Fig. 6

Late-p1 is severely affected in itga8 and fras1 single and double mutants. (A-D) Tissue sections labeled with anti-P63 (epithelial nuclei) and sox9a:EGFP (cartilage) at 72 hpf, and oriented as shown in A′. The late-forming outpocketing of pouch-1 (late-p1) is indicated with a yellow dotted line in wild type. However, late-p1 is absent in (B) itga8^b1161, (C) fras1^b1048, and (D) fras1^b1048;itga8^b1161 double mutants, leaving a large gap (blue line) between endoderm and ectoderm. (E) Symplectic cartilage length and ectoderm-endoderm gap distance were measured on confocal stacks of whole-mounted embryos expressing sox9a:GFP and labeled with anti-P63. Each measurement pair is plotted as a dot, with 95% density ellipses shown for each genotype.

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Acknowledgments

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Reprinted from Developmental Biology, 416(1), Talbot, J.C., Nichols, J.T., Yan, Y.L., Leonard, I.F., BreMiller, R.A., Amacher, S.L., Postlethwait, J.H., Kimmel, C.B., Pharyngeal morphogenesis requires fras1-itga8- dependent epithelial-mesenchymal interaction, 136-48, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.