ZFIN Image: Ji et al., 2016, Fig. S7

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Figure Caption

Fig. S7

Expression of pituitary markers in pitx2HD mutants. (A-C, E-G) Representative images of RNA in situ hybridizations of gh1 expression (A-C) and pomca expression (E-G) in wild-type (A, E;+/+), heterozygous (B, F;+/-) and homozygous mutant (C, G; -/-) pitx2HD^sny15 embryos at 5 dpf. A′-C′ and E′-G′ are enlarged views of boxed areas in A-C and E-G, respectively. s=somatotrope domain; c=corticotrope domain; m=melanotrope domain; hn=hypothalamic neurons. Dashed lines indicate expression domains that were quantified. (D, H) Quantification of the area of the gh1 expression domain (D) and pomca expression domains (H) in pitx2HD^sny15 embryos. *=significant difference (p<0.05) from wild-type using Student’s T-test. n=number of embryos analyzed. Supplementary material

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Reprinted from Developmental Biology, 416(1), Ji, Y., Buel, S.M., Amack, J.D., Mutations in zebrafish pitx2 model congenital malformations in Axenfeld-Rieger syndrome but do not disrupt left-right placement of visceral organs, 69-81, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.