ZFIN Image: Marquart et al., 2015, Fig. S5

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Figure Caption

Fig. S5

Synthetic 3′ UTR utr.zb1 suppresses transgene expression in muscle. The synthetic 3′ untranslated region utr.zb1 reduces expression in both slow and fast muscle, but not in heart or notochord. Epifluorescence from Kaede (green) and TagRFPT (red) iny323- Gal4, UAS:Kaede, UAS:epNTR-TagRFPT-utr.zb1 (A,B) or y274-Gal4, UAS:Kaede, UAS:epNTR-TagRFPT-utr.zb1 (C,D) embryos imaged at 3 dpf. In y323Et, Gal4 is expressed outside the brain in slow muscle, notochord, heart and caudal fin. Slow muscle (A, asterisk) expression is suppressed by utr.zb1 but expression in notochord (B, bracket), heart (arrowhead), and caudal fin remains intact. Similarly expression of Gal4 in fast muscle is suppressed by utr.zb1in y274Et (C, asterisk), but notochord expression (D, bracket) is not diminished.

Acknowledgments

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