ZFIN Image: George et al., 2016, Fig. 6

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Figure Caption

Fig. 6

PI(4,5)P₂ and PI(4)P distributions are not altered in nrc^a14 cones. A and B: The PI(4,5)P₂ probe GFP-PLCδ-PH was expressed in photoreceptors using the crx promoter. A: In wild type (WT) cells at 5 days post-fertilization (dpf), this probe localized to the plasma membrane and concentrated at synapses, but did not extend above the mitochondria (magenta) into the outer segment (OS). C: The PLC´-PH probe showed the same distribution in nrc^a14 photoreceptors from 3 to 6 dpf. PI(4)P was visualized with the probe RFP-FAPP1-PH. In both WT and nrc^a14 photoreceptors at 5 dpf, the RFP-FAPP1-PH probe (magenta) overlapped with the medial Golgi marker Man2a-GFP (green). There was no difference in the subcellular distribution of PI(4)P between WT and nrc^a14 photoreceptors. Scale bar = 2 µm in all images. IS, inner segment.

Acknowledgments

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