ZFIN Image: Scheid et al., 2016, Fig. 5

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Figure Caption

Fig. 5

Laz affects acto-myosin cooperativity and impairs calcium handling.

(A) pCa-force relationship, normalized to maximal force (at pCa 4.5). The sigmoidal curves were further analysed for pCa₅₀ and n_Hill. (B) Quantification of pCa₅₀ from the pCa-force relationship revealed no significant differences. (C) n_Hill was significantly increased for ^pslaz^+/- compared with wt, ^pswt, and laz^+/- (P < 0.05; N = 5). (D) Intracellular Ca²⁺-transients were analysed in isolated ventricular cardiomyocytes loaded with Fluo4-AM. Representative brightfield (left) and corresponding fluorescent (middle) and merged (right) images of a single ventricular adult zebrafish cardiomyocyte (scale bar 20 µm). (E) Representative Ca²⁺-transient traces recorded from wt, ^pswt, laz^+/-, and ^pslaz^+/-. (F) Peak area and (G) full-width half-maximum (FWHM) obtained from the analyses of Ca²⁺-transients. Data in (F) and (G) were statistically analysed by using ANOVA with Bonferonni post hoc pairwise multiple comparisons *P < 0.05; **P < 0.01; in A-C: N = 5; in E-G: n = 90 cells.

Acknowledgments

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