ZFIN Image: Lahvic et al., 2013, Fig. 7

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Figure Caption

Fig. 7

hspb7 knockdown disrupts cilia motility and asymmetric flow in Kupffer′s vesicle. (A) Analysis of cilia motility revealed that many cilia in hspb7 MO embryos were immotile or beat abnormally relative to controls. (B-C) Maximum projections of fluorescent bead movements superimposed on images of KV. Beads moved in a counterclockwise direction in control MO embryos (n=7/7 embryos; B), whereas this directional flow was absent in most hspb7 MO embryos (n=6/9 embryos; C). (D-F) Transmission electron micrographs showing: (D) transverse section of cilium from embryo injected with control MO showing 9+2 configuration of microtubules, (E) transverse section showing 9+0 configuration of microtubules in a cilium from embryo injected with hspb7 MO1+MO2, and (F) transverse section shows the abnormal central microtubule configuration in a cilium from embryo injected with hspb7 MO1. Arrows: outer dynein arms.

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Phenotype details

Acknowledgments

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Reprinted from Developmental Biology, 384(2), Lahvic, J.L., Ji, Y., Marin, P., Zuflacht, J.P., Springel, M.W., Wosen, J.E., Davis, L., Hutson, L.D., Amack, J.D., and Marvin, M.J., Small heat shock proteins are necessary for heart migration and laterality determination in zebrafish, 166-180, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.