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Fig. 5
The expression of zNanog in PGCs in 30% epiboly stage embryos. (I) zNanog and Vasa mRNA co-localized in the cytoplasm of PGCs in the 30% epiboly stage embryos by in situ fluorescence hybridization. zNanog and Vasa mRNA co-localized in the cytoplasm of PGCs in the 30% epiboly stage embryo cells. A and E: DAPI staining, B: Vasa RNA probes (green), C: zNanog RNA probes (red), F: Vasa sense probes, G: zNanog sense probes, D: merged of A-C, H: merge of E-G. Scale bars: 1.0 mm in A, B, C, D panel; 0.7 mm in E, F, G, H panel. (II) zNanog and zNanos co-localized in the PGC nuclei of the 30% epiboly stage embryos by fluorescent immunohistochemistry analysis. zNanog and zNanos co-localized in the nuclei of PGCs in the 30% epiboly stage embryos. A-D: zNanog and zNanos were stained using a primary polyclonal antibody against zNanog from rabbit (the secondary antibody: FITC-conjugated anti-rabbit) and against zNanos from mouse (the secondary antibody: Cy3-conjugated anti-mouse). A: DAPI staining, B: zNanog (green), C: zNanos (red), D: merge of A-C; E-H: The pre-immune sera from the rabbit and mouse were used as primary antibodies, and the signal was not observed. H: merge of E-G. Scale bars: 0.5 mm in all panels.