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Fig. 4
Effect of truncated Sek-1 on krx20 and rtk1 expression in the zebrafish hindbrain. RNA encoding truncated Sek-1 was microinjected into 1 cell of zebrafish embryos at the 2 cell stage. Embryos were allowed to develop to neurula stages, fixed and either krx20 or rtk1 expression analysed by in situ hybridisation. Photographs were taken either of the hindbrain from a dorsal (A-H,J) or lateral view (K,L), or after sectioning in the coronal plane (I). krx20 expression was analysed in (A) uninjected or (B,C) injected embryos fixed at 14 hours of development. rtk1 expression was analysed in uninjected (D) or injected (E-K) embryos analysed at 24 hours of development. (L) krx20 expression in injected 18h embryo. The arrowheads indicate cells expressing krx20 or rtk1 in even-numbered rhombomeres. Scale bars 50 µm.