ZFIN Image: Majumdar et al., 2000, Fig. 3

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Figure Caption

Fig. 3

Glomeruli differentiate at ectopic locations in flh. Wildtype (A, C, E) and flh (B, D, F) embryos hybridized with in situ probes: VEGF (A, B) at 40 hpf, flk-1 (C, D) at 40 hpf, and tie-1 (E, F) at 48 hpf. Differentiated podocytes express VEGF in wildtype embryos (A). VEGF-positive cells are found in ectopic lateral locations in flh (arrows in B). flk-1 labels glomerular capillary-forming endothelial cells in wildtype (C). flk-1-positive cells surround laterally positioned nephric primordia in flh (arrows in D). flk-1-positive cells are found in between nephric primordia and cardinal veins. tie-1 labels endothelia from cardinal veins (arrow in E) but not dorsal aorta in wildtype embryos at this time. In flh, tie-1-positive cells are associated with nephric primordia (arrows in F). Scale bar equals 50 µm.

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Acknowledgments

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Reprinted from Developmental Biology, 222(1), Majumdar, A. and Drummond, I.A., The zebrafish floating head mutant demonstrates podocytes play an important role in directing glomerular differentiation, 147-157, Copyright (2000) with permission from Elsevier. Full text @ Dev. Biol.