ZFIN Image: Shiu et al., 2016, Fig. 5

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Fig. 5

Morpholino-induced knockdown of YY1a results in down-regulation of PS receptor at 24 hpf. a Western-blot analysis of PSR proteins in lysates of YY1-MO-injected embryos. Note that PSR protein level in these embryos decreases in a YY1-MO dose-dependent manner (Fig. 5a, panel a, lane 1 [control-MO, 50 ng]; lane 2 [YY1-MO, 25 ng], and lane 3 [YY1-MO, 50 ng]). Panel b shows the actin loading control. b One-cell stage embryos were each injected with either 25 ng of control-MO or 25 ng of YY1-MO. At 12, 24, and 48 hpf, embryos were fixed and in-situ hybridization was performed as described in the Methods section. Phase-contrast images show normal development and psr expression patterns in wild type (panels a, d, and g) and control-MO-injected (panels b, e, and h) embryos but abnormal development (panels c, f, and i) and either a mild (panel c, 12 hpf) or severe (panel f, 24 hpf; panel i, 48 hpf) delay in psr expression (arrows) in YY1-MO-injected embryos. Bars indicate 100 µm

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Biomed. Sci.