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Fig. 3
smFISH provides quantitative spatial information on gene expression. Detected transcripts (magenta) and transcription foci (white) for sox19a (A), mex3b (B) and eif4g2a (C) at dome stage. Green, cell outlines; blue, nuclear outlines. Scale bars: 10µm. Images are maximum projections of 17 z-slices spaced by 0.3µm. (D) Tissue mask corresponding to C, to distinguish between EVL and DEL cells. (E) Quantification of transcript levels in DEL and EVL. Values are means from sections of three different embryos. Error bars represent s.e.m.
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