ZFIN Image: Wu et al., 2016, Fig. S2

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Figure Caption

Fig. S2

Spatially restricted gene expression reveals two distinct border zone regions. Related to Figure 3.

(A) In situ hybridization on consecutive heart sections (3 dpi) for genes identified by Tomo-seq to be upregulated in the BZmyo (nppb and mustn1) and BZinj (crip2 and ywhabl). Expression of crip2 and ywhabl overlapped with kdrl, a marker for endothelial cells. Scale bar, 50 µm. Dashed red line outlines the injury area.

(B) Expression traces from tomo-seq data (3 dpi) for genes involved in embryonic vascular endothelium development (cldn5b and mmrn2b), myocardial development (actc1 and desma) and cell cycle control (E2F1).

Acknowledgments

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Reprinted from Developmental Cell, 36(1), Wu, C.C., Kruse, F., Vasudevarao, M.D., Junker, J.P., Zebrowski, D.C., Fischer, K., Noël, E.S., Grün, D., Berezikov, E., Engel, F.B., van Oudenaarden, A., Weidinger, G., Bakkers, J., Spatially Resolved Genome-wide Transcriptional Profiling Identifies BMP Signaling as Essential Regulator of Zebrafish Cardiomyocyte Regeneration, 36-49, Copyright (2016) with permission from Elsevier. Full text @ Dev. Cell