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Fig. 2
In Situ Hybridization Validates Tomo-Seq Results
In situ hybridization for genes identified by tomo-seq to be enriched in the injury area (zone I) (A), wound border zone (zone II) (B), or the uninjured myocardium (zone III) (C) at 3 dpi (n = 3). Left: representative in situ staining; right: expression traces from tomo-seq data. y axis, read counts; x axis, section number. Scale bar, 100 µm. Dashed black line indicates wound boundary on sections. Red lines in plots surround the border zone.
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Reprinted from Developmental Cell, 36(1), Wu, C.C., Kruse, F., Vasudevarao, M.D., Junker, J.P., Zebrowski, D.C., Fischer, K., Noël, E.S., Grün, D., Berezikov, E., Engel, F.B., van Oudenaarden, A., Weidinger, G., Bakkers, J., Spatially Resolved Genome-wide Transcriptional Profiling Identifies BMP Signaling as Essential Regulator of Zebrafish Cardiomyocyte Regeneration, 36-49, Copyright (2016) with permission from Elsevier. Full text @ Dev. Cell