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Fig. 8
Figure 8. Expression of esco2 mRNA in WT and short fin (sof b123) fins by whole-mount in situ hybridization. WT and sof b123 fins were amputated at 50% level and allowed to regenerate for 5 days. At 5 dpa, the fins were harvested. At least six to eight fins were used per trial and 3 independent trials were performed. Left: Whole-mount in situ hybridization (ISH) on WT-5 dpa regenerating fins showing esco2 expression. Right: Whole-mount ISH on sof b123 -5 dpa regenerating fins shows similar expression levels of esco2. The brackets represent the zone of esco2 expression. Scale bar = 50 µm.
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