ZFIN Image: Khaliq et al., 2015, Fig. 1

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Figure Caption

Fig. 1

id2a expression in the developing liver. (A) WISH reveals id2a expression in the liver-forming region at 30 hpf (arrow) and in the liver at 48 hpf (arrow), 72 hpf and 5 dpf (dotted lines). From 72 hpf to, at least, 5 dpf, id2a expression in the liver appears to be restricted to BECs. Arrowhead points to the interrenal primordium; bracket denotes the intestinal bulb. Dorsal (30–72 hpf) or lateral (5 dpf) views, anterior to the left. (B–D) id2a in situ hybridization (red) combined with anti-GFP immunostaining (green) in Tg(prox1a:YFP) (B), Tg(Tp1:GFP) (C), or Tg(kdrl:GFP) (D) embryos reveals id2a expression in hepatoblasts at 36 hpf and BECs at 60 and 72 hpf, but not in the endothelial cells of the liver (dotted line), respectively. Asterisks mark id2a expression in the intestinal bulb. Single confocal section (B) or projections of z-stack confocal sections (C, D). Scale bars: 100 (A), 20 (B–D) µm.

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Expression / Labeling details

Acknowledgments

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Reprinted from Mechanisms of Development, 138 Pt 3, Khaliq, M., Choi, T.Y., So, J., Shin, D., Id2a is required for hepatic outgrowth during liver development in zebrafish, 399-414, Copyright (2015) with permission from Elsevier. Full text @ Mech. Dev.