ZFIN Image: Teh et al., 2015, Fig. 2

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Fig. 2

Spatio-temporal expression of wnt3 promoter-driven EGFP/Wnt3EGFP. Spatio-temporal expression of 4kb wnt3 promoter-driven EGFP/Wnt3EGFP influenced by genomic insertion of a transgene varies in strength. Tg(-4.0wnt3:EGFP)^F1, Tg(-4.0wnt3:Wnt3EGFP)^F1, Tg(-4.0wnt3:Wnt3EGFP)^F2 and Tg(-4.0wnt3:Wnt3EGFP)^F3 are abbreviated in the figure as Tg(wnt3:EGFP)^F1, Tg(wnt3:Wnt3EGFP)^F1, Tg(wnt3:Wnt3EGFP)^F2 and Tg(wnt3:Wnt3EGFP)^F3, respectively. (A-O) Initial spatio-temporal similarity of expression of EGFP and Wnt3EGFP is maintained in three independent transgenic lines. (P-W) Tg(-4.0wnt3:Wnt3EGFP)^F3 maintains strong Wnt3EGFP expression in the cerebellum beyond 48hpf. Transgene expression in the optic tectum and cerebellum decreases from 48hpf in all other Wnt3 transgenics. 4^th ven, fourth ventricle; ce, cerebellum; ot, optic tectum; rp, roof plate.

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