ZFIN Image: Teh et al., 2015, Fig. S9

Image

Figure Caption

Fig. S9 Effect of C59 Wnt inhibitor on brain patterning. Exposure of Tg(wnt3:Wnt3EGFP)^F2 to C59 Wnt inhibitor suppresses Wnt3EGFP expression and interferes with brain patterning. Dorsal domains of the brain are highlighted by the expression of membrane tethered KillerRed in the epithalamus, optic tectum, cerebellum and hindbrain of Tg (memKR15-8). Double transgenic larvae were treated with 5 µM C59; 50 µM IWR-1 or 1% DMSO from 10 hpf to 48 hpf. Successful interference of Wnt function by C59 results in decreased Wnt3EGFP expression that correlated with defective brain patterning indicated by the absence of epithalamus (ep), optic tectum (ot) and cerebellum (ce) in C59 treated larvae (D).

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