ZFIN Image: Hamling et al., 2015, Fig. 5

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Figure Caption

Fig. 5

Purkinje cell dendritic arbor develops over time. (A) Pvalb7 and VGlut1 expression in Purkinje cells at 3.3 dpf (80 hpf). Pvalb7 (A1), Vglut1 (A2) expression, and merge of channels (A3) shows that Pvalb7 is expressed in cell bodies but no visible primary dendrites, and that VGlut1 is found directly dorsal to Purkinje cell bodies and overlapping with some possible Pvalb7 expression in neuropil (white dashed region). (B1–3) By 5dpf, the immature dendrites are also overlapping with the expanded VGlut1 expression in parallel fibers. (C1–3) By 7dpf, the Purkinje cell dendrite has grown dorsally and the VGlut1 layer has expanded accordingly to remain overlapping. Inset in A2 shows rough location of sagittal plane of section (indicated by dashed line). Scale bar 10 µm. Images taken using 20× (1.0 NA) water objective, at 5× zoom.

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Expression / Labeling details

Acknowledgments

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