Fig. 5
HS2 exhibits higher yields of gynogenetic diploids than standard Heat Shock. A: In three independent trials, pools of eggs were divided and treated with standard Heat Shock (HS) or HS2. HS2-treated embryos exhibit consistently higher yields of gynogenetic production. Bars indicate the standard error in average yield values. A chi-square test for equality of proportions performed on the combined data shows a significant difference in the average yield values between standard Heat Shock and HS2 (P-value = 0.016; Χ2 = 5.8). Control untreated embryos resulted in 100% embryos with haploid morphology. B,C: HS2-derived gynogenetic adult offspring from golden (B) and casper (C) mutant lines.