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Fig. 6 Wnt9b morphants exhibit disrupted Wnt3a and Wnt5b expression domains. Anterior to left. Left column is Wnt3a expression, right column is Wnt5b expression. (a) MM control (n = 14) and (b) morphant embryos (n = 14) were aged to 28hpf and stained with Wnt3a riboprobe. 71% of morphant embryos exhibited expanded Wnt3a expression in the brain compared to controls. (c) MM control (n = 10) and (d) morphant embryos (n = 9) were aged to 28hpf and stained with Wnt5b riboprobe. 100% of morphant embryos exhibited expanded Wnt5b expression in the tail with reduced Wnt5b expression in the pharyngeal arches compared to controls. (a), (b) Red arrowheads indicate brain expression, black arrows indicate rhombic lip expression. (c), (d) Red arrows indicate the PAs, white arrowheads indicate the fin buds, black arrowheads indicate the tail.