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Fig. 5 Wnt9b morphants exhibit severely reduced dlx2a expression in pharyngeal arches 1 and 3-7. (a)-(d) Anterior to right, top row = dorsal view, bottom row = lateral view. MM control (n = 17) (a), (c) and morphant (n = 20) (b), (d) embryos were aged to 24hpf and stained with dlx2a antisense riboprobe. 60% of morphant embryos exhibited reduced dlx2a expression in the pharyngeal arches compared to controls. Red arrowheads indicate forebrain. Black arrows indicate PA1, black arrowheads indicate PA2, white arrowheads indicate PA3-7. Note that dlx2a expression is mostly reduced in PA1 and PA3-7. Br = brain, E = eye. Size bars = 100 µm.