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Fig. 3, S1
Validation of endocrine differentiation induction: precocious 2° islet assay (neurod reporter).
Precocious 2° islet assays were performed as per Figure 3. (A-J) Representative confocal images - brightfield and fluorescence images merged - of dissected pancreata (dashed lines) from neurod:EGFP transgenic larvae treated with indicated Lead I compounds (at optimal concentrations) from 3 to 5 dpf: (A) Thioctic acid (5 µM); (B) N-acetylmuramic acid (12.5 µM); (C) Maprotiline (5 µM); (D) β-estradiol (12.5 µM); (E) Biperiden HCl (12.5 µM); (F) Benzamidine (20 µM); (G) Dexetimide (1 µM); (H) Oxacillin (5 µM); (I) Dimethindene (S, +) maleate (1 µM); (J) Methylthiouracil (12.5 µM). Secondary islets are indicated by arrows. Scale bar, 25 µm.