ZFIN Image: Liu et al., 2013, Fig. 2

Image

Figure Caption

Fig. 2 Zfyve9a is required for liver development. (A) A cartoon of the zfyve9a gene structure shown the positions of MO-AUG, MO-SP and primers used in RT-PCR reaction in (C). (B) MO-AUG (4 ng) effectively inhibited the translation of Zfyve9a-GFP mRNA (100 pg) as determined at about 70% epiboly stage. (C) RT-PCR analysis for the effect of MO-SP. PCR products from wild type and zfyve9a morphants were cloned and sequenced. The lower band in MO-SP treated sample corresponds to a deletion of the C-end of exon 2. (D) Representative live images of wild type embryo and zfyve9a morphants at day 3. (E-F) Expression of fabp10a in WT, MO-AUG or MO-SP injected embryos at day 3 (E) and day 4 (F). (G) Expression of another liver marker, cp, in WT embryos and zfyve9a morphants at day 4. (H-J) Knockdown of zfyve9a did not affect the development of pancreas or gut at day 4. insulin labels the pancreatic beta-cells, trypsin indicates the exocrine pancreas and fabp2 marks the intestine.

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Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Dev. Biol.