ZFIN Image: Hall et al., 2013, Fig. 1

Image

Figure Caption

Fig. 1

Macrophage-Lineage Cells Express irg1 in Response to Infection

(A) Temporal QPCR analysis of irg1 expression within infected larvae (relative to PBS-injected controls) throughout first hour of infection, n = 3 biological replicates (mean ± SD).

(B and C) Expression of irg1 within PBS-injected control and infected larvae at 1 hpi, respectively. Arrow marks hindbrain ventricle injection site. Inset, magnified view of boxed region of similarly treated larva (10× objective).

(D) Quantification of irg1⁺ cells within individual infected larvae at 10, 20, 30, 40, 50, and 60 mpi (mean ± SD).

(E and F) Expression of irg1 within infected larvae at 6 and 24 hpi, respectively.

(G–I) Dual expression analysis of irg1 and mpeg1 (G), apoeb (H), and lyz (I) within the midbrain/hindbrain region of infected larvae at 24 hpi.

(J and K) Expression of irg1 within infected control MO-injected and Irf8-depleted larvae, respectively, at 1 hpi.

(L) Quantification of irg1⁺ cells within individual infected larvae as shown in (J) and (K) (mean ± SD). All views anterior to left. Numbers represent frequency of larvae with displayed phenotype. Scale bar, 100 µm in (B). p < 0.0001. See also Figure S1.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

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Reprinted from Cell Metabolism, 18(2), Hall, C.J., Boyle, R.H., Astin, J.W., Flores, M.V., Oehlers, S.H., Sanderson, L.E., Ellett, F., Lieschke, G.J., Crosier, K.E., and Crosier, P.S., Immunoresponsive Gene 1 Augments Bactericidal Activity of Macrophage-Lineage Cells by Regulating β-Oxidation-Dependent Mitochondrial ROS Production, 265-278, Copyright (2013) with permission from Elsevier. Full text @ Cell Metab.