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Fig. 4
Generation of Transgenic, Tissue-Specific Knockout Fish
Confocal images of 30 hpf embryos of the LCR:GFP transgenic line and F1 generation of fish stably expressing pcmlc2:GFP, U6:gRNA urod, gata1:Cas9 vector. See also Figure S4A.
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Acknowledgments
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Reprinted from Developmental Cell, 32(6), Ablain, J., Durand, E.M., Yang, S., Zhou, Y., Zon, L.I., A CRISPR/Cas9 Vector System for Tissue-Specific Gene Disruption in Zebrafish, 756-64, Copyright (2015) with permission from Elsevier. Full text @ Dev. Cell