ZFIN Image: Paik et al., 2013, Fig. 5

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Figure Caption

Fig. 5

Overexpression of scl and lmo2 Rescues the Loss of RBCs in the cdx4^mo/sall4^mo Embryos

(A) DNA-mediated overexpression scheme using Tg(lcr:GFP) embryos that express GFP in globin+ RBCs. When the embryos reached the 18-somite stage, mCherry+ embryos were selected and their GFP expression and gata1 expression were examined.

(B) cdx4^mo/sall4^mo/Tg(lcr:GFP) embryos that were control plasmid injected (top), ubi:scl injected (middle), or ubi:scl; ubi:lmo2 injected (bottom). The mCherry+ cells show the mosaicism. Note the GFP+ cells in the ICM (white arrow) in the bottom row. The numbers indicate the number of embryos with GFP signal in the ICM.

(C) gata1 WISH of embryos shown in (B). The numbers indicate the number of embryos with gata1 signal in the ICM.

All scale bars, 200 µm. See also Figure S5.

Acknowledgments

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