Image
|
Figure Caption
Fig. 7
Dynasore treatment does not cause major defects in EVL specification or apoptosis. ((A)(C)) Expression of keratin 4 (krt4), a marker of EVL differentiation, in untreated (A), DMSO control treated (B) and dynasore treated (C) embryos. Arrowheads in (C) indicate regions with reduced krt4 staining. ((D)(F)) Co-staining of untreated (D), DMSO treated (E) and dynasore treated (F) embryos with phalloidin and anti-cleaved Caspase 3, a marker of apoptotic cell death. Few Caspase 3-positive cells are observed in (F). Inset in (F) shows that these cells are not incorporated in the EVL.
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and
ZFIN has permission only to display this image to its users.
Additional permissions should be obtained from the applicable author or publisher of the image.
Reprinted from Developmental Biology, 385(1), Lepage, S.E., Tada, M., and Bruce, A.E., Zebrafish Dynamin is required for maintenance of enveloping layer integrity and the progression of epiboly, 52-66, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.