ZFIN Image: Gu et al., 2015, Fig. 1

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Figure Caption

Fig. 1

(A) S1P₂ immunoblot of HBE cells expressing control (left) or S1P₂-specific shRNA (right) with α-tubulin as loading control. (B) Representative images of HBE cells (DNA only) expressing control (left) or S1P₂-specific shRNA (right) grown for 3 weeks. Scale bar, 10 µm. (C) Proliferation assay indicates that S1P₂-knockdown cells proliferate at the same rate as wild type controls cells. (D) Representative DIC micrographs of 5-dpf WT (top) and Mil (S1P₂ mutant) (bottom) zebrafish larvae, where cartoon shows region where fish was imaged. Scale bars, 100 µm where red box indicates region imaged. (E) Quantification of epidermal clumps of 22 zebrafish larvae.

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Phenotype details

Acknowledgments

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