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Fig. S9 Double WISH analysis of ectopic gene expression in the pronephros of fluid flow and cardiac contractility morphants. WISH was used to assess gene expression using antisense riboprobes to detect the combination of (A) wt1a (purple) and cdh17 (red) transcripts in (A) wild-types, (B) prkCI/ζ morphants, (C) ift88 morphants, and (D) tnnt2a morphants. Only prkCI/ζ morphants displayed overlapping expression of wt1a in cdh17-expressing nephron tubule cells.
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Reprinted from Developmental Biology, 396(2), Gerlach, G.F., Wingert, R.A., Zebrafish pronephros tubulogenesis and epithelial identity maintenance are reliant on the polarity proteins Prkc iota and zeta, 183-200, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.