Fig. S7

Fig. S7 prk_CI/ζ deficient embryos display reduced protein expression of the Na⁺/K⁺ ATPase in the neck region of the pronephros. Based on whole mount IF, wild-type embryos at 36 hpf exhibited Na⁺/K⁺ ATPase protein expression along the entire length of the pronephric tubule, including the proximal-most region (white bar). In contrast, double prk_CI/ζ morphants at the 36 hpf stage fail to show Na⁺/K⁺ ATPase in the proximal most region of the pronephros (line of white dots). In wild-type embryos at 72 hpf, Na⁺/K⁺ ATPase continues to demarcate the entire tubule and shows the convoluted nature of the PCT (white bar), while prk_CI/ζ deficient embryos have linear tubules that have failed to undergo normal morphogenesis. (Inset) Regions of the PCT in prk_CI/ζ morphants exhibit folds and bulges (white arrowheads).