Fig. S4

Fig. S4 Expression of odf3b and acetylated α-tubulin in wild-type control and prk_CI/ζ deficient embryos. WISH was used to evaluate the gene expression of odf3b (purple), a ciliogenesis marker, in wild-types (left top, middle panels) and double prk_CI/ζ morphants (right top, middle panels) at the 36 hpf stage. Wild-type embryos and double prk_CI/ζ knockdowns formed similar numbers and arrangements of odf3b-expressing cells, though occasional ectopic odf3b+ cells were observed in the proximal region of the pronephros in prk_CI/ζ morphants (boxed area, top right panel, which is enlarged in the middle right panel). Asterisk indicates region where ectopic odf3b+ cells were located. (Bottom left, right panels) IF performed on cross sections of wild-types and double prk_CI/ζ morphants at the 22–24 ss revealed that acetylated α-tubulin (light blue) was similarly present at the apical membrane of pronephros cells (labeled by GFP (green), nuclei marked with DAPI (dark blue), although double prk_CI/ζ morphants lacked Prkc (red).