ZFIN Image: Hartwig et al., 2014, Fig. 1

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Figure Caption

Fig. 1

Zebrafish regulator of G-protein signaling 14a (rgs14a) RNA expression and Rgs14a protein localization. (A) A schematic representation of the Rgs14a protein structure showing the N-terminal RGS domain, the two Raf-like Ras-binding domains (RBD), and the C-terminal GoLoco domain. (B) Whole-mount in situ RNA hybridization using rgs14a (Left) and nanos (Right) antisense RNA probes at the indicated embryonic stages; 5.3- and 10-hpf embryos were overstained in the case of rgs14a in situ hybridization to detect the weak expression in the PGCs. (C) GFP-Rgs14a fusion protein expressed in PGCs is localized to the cytoplasm and the plasma membrane, with enrichment in perinuclear granules (in 91% of the 46 PGCs analyzed; Top) or in the nucleus (in 9% of the 46 PGCs analyzed; Middle). The perinuclear granules where the GFP-Rgs14a protein is localized harbor Vasa-DsRed protein (Bottom), defining them as germ cell granules. (Scale bars, 10 μm.)

Figure Data

Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA