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Fig. 1
Generation of zebrafish with nuclear Hh pathway reporters.
(A) Design of the Gli-dependent nuclear mCherry reporter constructs. (B–C) Tg(8xGliBS:mCherry-NLS) and Tg(8xGliBS:mCherry-NLS-Odc1) larvae at 84 hpf. Fluorescence and brightfield images of representative zebrafish are shown, and the telencephalic nuclei (tn), optic tectum (ot), cerebellum (c), and hindbrain (hb) are labeled. Embryo orientations: lateral view and anterior left. Scale bars: 200 μm.
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