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Fig. S19
Differentiated thymic T-cells could be readily identified by rag1 expression when examined at 4 dpf. Whole mount in situ hybridization with a rag1 riboprobe revealed a severe decrease in the number of T lymphocytes in kalrn2, mfn2, pdia5 and psmd13 MO injected embryos in thymi (arrow) at 4 dpf. Knock down of akap10, kalrn1 and rcor1 caused a moderate decrease in T lymphocyte numbers. All the embryos are positioned anterior up and dorsal to the back.
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