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Fig. 10
The small liver phenotype caused by hif2-alpha knockdown can be rescued by ectopic leg1 expression.
The expression pattern of the lfabp gene was examined in Tg(lfabp:EGFP) embryos (A) and compared with hif2-alpha ATG-MO-injected embryos (8 ng) (B) as well as embryos co-injected with hif2-alpha ATG-MO (8 ng) and leg1 cRNA (C). (D) lfabp mRNA expression was detected by qPCR in wild-type embryos and compared with hif2-alpha ATG-MO-injected embryos, hif2-alpha ATG-MO and leg1 cRNA co-injected embryos, as well as hif2-alpha ATG-MO and hif2-alpha cRNA co-injected embryos. Expression was normalized to β-actin.
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