ZFIN Image: Carr et al., 2014, Fig. 8

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Figure Caption

Fig. 8

Nucleo-cytoplasmic shuttling of zebrafish ZIPK. HeLa cells were transfected with either human GFP-ZIPK (A–F); rat GFP-ZIPK (G–L); or zebrafish GFP-ZIPK (M–R). All cells were fixed and stained with DAPI and Alexa 568-phalloidin. The cells in (A–C, G–I, M–O) were treated with 0.1% DMSO; while the cells in (D–F, J–L, P–R) were treated with 50 nM leptomycin B for four hours. (A, D, G, J, M, P) are stained with DAPI; (B, E, H, K, N, Q) show subcellular localization of the GFP-fusion protein, while color images are a merge of DAPI (blue), GFP (green), and phalloidin (red). Each experiment was repeated three times with a minimum of 25 cells assayed per experiment. White bar indicates 20 μm.

Acknowledgments

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