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Fig. 6
Insulin abolishes autophagy in cells with RPS19 loss.
(A) Confocal microscopy analysis of GFP-LC3 HEK cells transfected with siScr or siRPS19 and either untreated or stimulated with 350 nM insulin for 6 hours. Size bars = 10 μM. (B) Quantification of the average number of GFP-LC3 puncta per cell in (A). (C) Western blot analysis of LC3 expression in cells from (A). Densitometer analysis used to calculate the ratio of LC3II/actin.
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