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(A, B) H+ flux during regeneration after vivo-MO mediated atp6v1e1b knockdown, 2 h after proximal (A) and distal (B) amputation. *statistical significant results (p<0.05). (C, D) Caudal fin regeneration after atp6v1e1b vivo-MO delivery to atp6v1e1bhi577aTg/+ (AB) fish, 2 h after proximal amputation (C) and 16 h after distal amputation (D). (C′, D′) Percentage of regenerate area in atp6v1e1b knocked down regions compared to the control vivo-MO (cVivo-MO). Vivo-MOs were delivered 2 h (C′) or 16 h (D′) after both proximal and distal amputation. hpa: hours post amputation. For each panel, n = 8.
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