ZFIN Image: Govindan et al., 2014, Fig. 5

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Figure Caption

Fig. 5

Morpholino mediated knock-down of hapln1a in WT regenerating fins results in reduced HA levels.

HA is detected by biotin-HABP with streptavidin-Alexa-546. HOECHST detects DNA (blue). The green reveals the location of the targeting and control MOs, which are fluorescein tagged. (A,B) Fin sections from 5 dpa WT regenerating fins were either untreated or treated with hyaluronidase. The reduced signal in the hyaluronidase-treated sections demonstrates that HABP detects HA. (C) Longitudinal section of a fin ray treated with Hapln1a control morpholino (MM). (D) Longitudinal section of a fin ray knocked down for Hapln1a with a targeting morpholino (KD). Compared to the control MM fins, Hapln1a knock-down (KD) fins exhibit reduced staining for HA. Arrow identifies the basal layer of the epidermis; m, mesenchyme; e, epithelium. Scale bar is 50 μm.

Acknowledgments

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