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Fig. S2
Images confirming the presence of SGs. (A) Heat-shocked cells treated prior with the SG inhibitor cycloheximide (CHX) did not form FUS-GFP containing SGs. (B) Islet1: GFP cells did not form SGs after heat-shock, indicating that the GFP tag was not involved in SG formation. (C) Non-GFP fused cultures of FUS-R521C counterstained with anti-human FUS show the presence of aggregates of similar appearance to SGs tagged with GFP. (D) The majority of non-transgenic cells did not form SGs that could be stained with anti-FUS. Scale bars = 10 μm.
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