Fig. 4
Loss of Cyp26 enzymes leads to differential shifts in cardiac progenitor markers. (A-B2) Double in situ hybridization of nkx2.5 (red) and etv2 (blue) shows a small gap between expression domains in Cyp26-deficient embryos that is not present in wild-type siblings. (C-D2) Double in situ hybridization with hand2 (blue) and etv2 (red) shows no space between expression domains in wild-type and Cyp26-deficient embryos. A-D are dorsal views with anterior upwards. A2-D2 are dorsal views with anterior leftwards. A2-D2 correspond to boxed areas in A-D, respectively. Arrows indicate the borders between expression domains.