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Fig. 6 A cell-autonomous role for Nhsl1b, Scrib and Vangl2 in migration. (A-J) Live confocal images at 48 hours post-fertilization (hpf) of chimeric zebrafish embryos with anterior to the top. Cascade blue marks donor-derived cells (blue), Tg(isl1CREST-hsp70l:mRFP)fh1 marks host motorneurons (red) and Tg(isl1:GFP) marks donor-derived motorneurons (green). Histograms on the right indicate the percent of donor-derived FBM neurons in rhombomere (r)4 (unmigrated), r5 and r6 (fully migrated) under the transplantation conditions indicated on the far left, which are written as Donor>Host. n refers to the total number of FBM neurons scored in each condition. Pk1b MOs were used in D, E, G and I to prevent host FBM neurons from migrating by a cell-autonomous mechanism. J shows the rescue of host nhsl1b mutant FBM neurons expressing Tg(isl1:GFP) (green) (arrow) by wild-type donor FBM neurons expressing Tg(isl1CREST-hsp70l:mRFP)fh1 (red). Scale bar: 50 µm.