Fig. 2
Sulf1 depletion impairs generation of ventral neuronal subtypes. Side views of whole embryos at the level of the trunk spinal cord, anterior to the left and dorsal to the top. (A-H) Detection (A-F) and quantification (G,H) of tal2-expressing neurons at 24 hpf in embryos injected with ctrlMO (A), sulf1 mismatch MO (B) and sulf1MO (C) and in wild-type (D), sulf1sa199+/- (E) and sulf1sa199-/- (F) embryos. (I-Q) Detection and cell counts of sim1+ (I-K), islet2a+ MNs (L-N) and pax2a+ dorsal neurons (O-Q) in embryos injected with ctrlMO (I,L,O) and sulf1MO (J,M,P). Results are presented as mean number of cells ± s.e.m. (*P<0.05, **P<0.01). Scale bars: 100 μm.