Fig. S1

Fig. S1 Knockdown/mutation of Delta family members affects V2 interneuron development. (A, E) DeltaA and DeltaD are redundant for p2 progenitor maintenance. Both V2a and V2b cells were increased and expanded into the ventricular zone in deltaA/D double mutants. Control (sib), deltaA mutant (dla^dmc72a), deltaD mutant (dld^tr33), deltaA/D double mutants (dla^dmc72a; dld^tr33). (B, C, F, and G) DeltaA and DeltaC are redundant for V2a/V2b cell fate determination. V2a cell numbers were increased, whereas V2b cell numbers were not dramatically changed, and their localization was altered to the ventricular zone in deltaA/C double knockdown embryos. (C, G) Mutation in deltaC combined with deltaA knockdown led to increased V2a cell numbers and comparable V2b cell numbers (100%, vsx2, n=21; 100%, sclα, n=22). (D, H) DeltaC and DeltaD are redundant for p2 progenitor maintenance. Mutation in deltaC combined with deltaD knockdown resulted in increased V2a and V2b cell numbers (100%, vsx2, n=8; 100%, sclα, n=7). Homozygous deltaC^tit446 mutant embryos were injected with control or deltaA MO (C, G) or with deltaD MO (D, H). V2a and V2b cells were detected by vsx2 (A–D) and sclα (E–H), respectively. Left four panels (in A–H): side views of embryos at 24 hpf with anterior to the left, dorsal up. Dorsal and ventral borders of the neural tubes are shown by the dotted lines. Bar scale: 50 µm. Right two panels (in A, B, E, and F): transverse sections through the trunk region in embryos at 24 hpf. The neural tubes are circled by white dotted lines. Bar scale: 20 µm.