Fig. 1

Fig. 1 The smu mutant phenotype is due to mutations in smoothened. (A) 24 h wild type (top); 24 h smu^b641 mutant (bottom). (B) In smu^b641 mutants (bottom), floor plate was poorly differentiated, somites lacked a horizontal myoseptum and were U-shaped compared with wild type (top). (A,B) Side views, dorsal is towards top, anterior is towards left. Scale bar: 300 μm in A; 50 μm in B. (C) Phylogenetic analysis. Statistical robustness was estimated by bootstrap, numbers indicate the percent of times each node was obtained. Danio rerio (Dre), Drosophila melanogaster (Dme, P91682), Homo sapiens (Hsa, NP_005622), Gallus gallus (Gga, O42224), Mus musculus (Mmu, P56726) and Rattus norvegicus (Rno, NP_036939). (D) Syntenic relationships. Conserved syntenies to Hsa 7q32.3 indicate that smoothened is a zebrafish ortholog of mammalian smoothened. (E) Mapping. Diagram of LG4 showing positions of smoothened (smoh) gene and smu mutations. (F) Model of protein encoded by smoothened. The red (smu^b641) and green (smu^b577) dots indicate amino acids altered in the two alleles. A seven-pass transmembrane protein is the predicted structure of zebrafish Smoothened (TMHMM1.0 software, Center Biol. Sequence Analysis, Department Biotech., Tech. University of Denmark). (G) Mutations. Point mutations in smu^b641 (red) and smu^b577 (green).