Fig. 2

Fig. 2 nr2f2 function is required for proper venous gene expression in the zebrafish vasculature. (A–D) Transmitted light (A and B) and green fluorescent light (C and D) images of control (A and C) and nr2f2 morpholino injected (B and D) Tg(fli1a-EGFP)^y1 transgenic animals. (E–L) Whole mount in situ hybridization of 24 hpf control (top) and nr2f2 morpholino injected (bottom) zebrafish embryo trunks probed for ephb4a (E), stab1 (F), lyve1 (G), sox7 (H), efnb2a (I), grl (J), tbx20 (K), and sox18 (L). Blue arrowheads note posterior cardinal vein gene expression, while red arrows note dorsal aorta gene expression. The inset numbers in panels F–L show the number of in situ-stained nr2f2 MO-injected embryos exhibiting the phenotype shown in the image panel over the total number of embryos examined. (M) Quantitative RT-PCR measurement of gene expression in excised trunks (see Materials and Methods) of 24 hpf control and nr2f2 morpholino injected animals. Values are all normalized to control gene expression levels, which are set equal to 1. Rostral is to the left and dorsal is up in all image panels. Scale bars, (A and B) 500µm, (C–L) 100µm.