ZFIN Image: Choe et al., 2014, Fig. 3

Image

Figure Caption

Fig. 3

TALE Factors Recruit RNA Polymerase II but Drive Only Weak hoxb1a Transcription

(A–I) In situ hybridization to hoxb1a transcripts in 4 hpf zebrafish embryos injected with mRNA and MOs as indicated in lower right corner of each panel. Arrows indicate examples of positive signals.

(J and K) Quantitative PCR detection of hoxb1a transcripts in 4 hpf zebrafish embryos injected with the indicated mRNAs and MOs.

(L) Occupancy of serine-5 phosphorylated RNA polymerase II at the hoxb1a promoter of 4 hpf embryos overexpressing TALE factors or TALE factors + Hoxb1b.

Data are presented as the average of a minimum of three repeats (except in K, which was done in duplicate) with error bars indicating SD. Statistical significance was determined using Student’s t test in Microsoft Excel; p values < 0.05 are indicated.

Figure Data

Expression / Labeling details

Acknowledgments

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Reprinted from Developmental Cell, 28(2), Choe, S.K., Ladam, F., and Sagerström, C.G., TALE factors poise promoters for activation by Hox proteins, 203-211, Copyright (2014) with permission from Elsevier. Full text @ Dev. Cell